Decision making in groups : theory and practice

The purpose of this chapter is twofold: i) to focus attention descriptively on some important aspects of decision making in teams - namely; the sense making process, the nature of multiple perspectives and interpretations, the interdependency of problems that make up a difficult and messy situation, the nature of purposeful behaviour - goals systems, and perspectives on psychological and social negotiation; and ii) to explore ways of translating these descriptions into prescriptions that might reduce the probability of decision failures. The description of decision making and problem solving processes presented in the chapter links directly with some of the aspects of decision making that might decrease the probability of failures. Theories from psychology, social psychology, group processes, the nature of problems and psychological and social negotiation are used as a basis for description. The chapter introduces cognitive and causal mapping as a method for describing and managing decision situations including: sense making, multiple perspectives, and goals systems. The chapter also considers the use of causal mapping as a tool for facilitating negotiation in teams and so aiding the reaching of agreements about how to act. In addition the chapter discusses the role of group support systems (GSS) and operational research in addressing aspects of decision failure. In particular the use of a GSS is argued to address delivering a balance between the potential benefits and potential dangers accrued from drawing multiple stakeholders into decision making situations. The chapter draws particularly upon the research of Nutt that led to his analysis of failed decisions (Nutt, 2002)

Discovering and Locating High-Energy Extra-Galactic Sources by Bayesian Mixture Modelling

Discovering and locating gamma-ray sources in the whole sky map is a declared target of the Fermi Gamma-ray Space Telescope collaboration. In this paper, we carry out an unsupervised analysis of the collection of high-energy photons accumulated by the Large Area Telescope, the principal instrument on board the Fermi spacecraft, over a period of around 7.5 years using a Bayesian mixture model. A fixed, though unknown, number of parametric components identify the extra-galactic emitting sources we are searching for, while a further component represents parametrically the dffuse gamma ray background due to both, extragalactic and galactic high energy photon emission. We determine the number of sources, their coordinates on the map and their intensities. The model parameters are estimated using a reversible jump MCMC algorithm which implements four different types of moves. These allow us to explore the dimension of the parameter space. The possible transitions remove from or add a source to the model, while leaving the background component unchanged. We furthermore present an heuristic procedure, based on the posterior distribution of the mixture weights, to qualify the nature of each detected source

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach

People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University of St Andrews.Single-stranded DNA binding proteins (SSBs) are ubiquitous across all organisms and are characterized by the presence of an OB (oligonucleotide/oligosaccharide/oligopeptide) binding motif to recognize single-stranded DNA (ssDNA). Despite their critical role in genome maintenance, our knowledge about SSB function is limited to proteins containing multiple OB-domains and little is known about single OB-folds interacting with ssDNA. Sulfolobus solfataricus SSB (SsoSSB) contains a single OB-fold and being the simplest representative of the SSB-family may serve as a model to understand fundamental aspects of SSB:DNA interactions. Here, we introduce a novel approach based on the competition between Förster resonance energy transfer (FRET), protein-induced fluorescence enhancement (PIFE) and quenching to dissect SsoSSB binding dynamics at single monomer resolution. We demonstrate that SsoSSB follows a monomer-by-monomer binding mechanism that involves a positive-cooperativity component between adjacent monomers. We found that SsoSSB dynamic behaviour is closer to that of Replication Protein A than to Escherichia coli SSB; a feature that might be inherited from the structural analogies of their DNA-binding domains. We hypothesize that SsoSSB has developed a balance between highdensity binding and a highly dynamic interaction with ssDNA to ensure efficient protection of the genome but still allow access to ssDNA during vital cellular processes.Publisher PDFPeer reviewe

Sun protective behaviour and sunburn prevalence in primary and secondary schoolchildren in western Switzerland.

Although solar overexposure during childhood and adolescence increases the risk of melanoma, determinants of sunburn and sun protective behaviours of Swiss children have scarcely been explored. We investigated sunburn occurrence and sun protective behaviours of schoolchildren in western Switzerland, the region with the highest incidence of melanoma in Europe. Self-reported questionnaires were administered during regular classes to pupils in 5th (primary school, n = 431), 8th and 11th grades (secondary school, n = 837) in the 18 public schools of La Chaux-de-Fonds. Descriptive statistics and multivariate logistic regression analyses were performed to assess predictors of sunburns and of three sun protective behaviours (sunscreen, shade, wear of covering clothes). Response rate was 91%. Sunburn prevalence over the preceding year was high (60% at least one sunburn, 30% at least two, 43% at least one severe sunburn). Younger age, fair skin, regular sunscreen use, higher sun-related knowledge and preference for a tanned skin were predictors of sunburn. Sunscreen was the most used protective measure (69%), followed by seeking shade (33%) and wearing long-sleeved shirts (32%). Decline in all protective measures was observed in older pupils and those with pro-tan attitudes. The wear of covering clothes was significantly associated with sunscreen use and seeking shade. Parental encouragement favoured sunscreen use and wearing of protective clothes. Sunscreen use as a last protective barrier against ultraviolet radiation should be better emphasised in prevention campaigns targeting children and adolescents. Multi-faceted interventions, including role models, parents and peers should help to improve children's sun protective behaviours